Abstract

A new method for the quantification of velocity of movement of single muscle cells in culture is described. The method involves television monitoring of microscope images and video analysis to yield data in chart form. Recorder printout is linearly related to distance of cell movement. Two new modifications of techniques for measuring the number of muscle cell movements are also described. These include television video analysis and a light-sensitive field effect transistor (Photo-fet). Application of both techniques to the measurement of contractions of single beating heart cells is shown, and the chronotropic effect of norepinephrine is monitored with increasing concentrations of the drug. Application of video analysis to the inotropic effect of the same drug also is studied, and quantitative data for velocity of contraction and relaxation are determined in microns per second.

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