Measurement of the magnesium concentration in perennial ryegrass (Lolium perenne) using near infrared reflectance spectroscopy

Abstract

Near infrared reflectance spectroscopy (NIR) was used to develop calibration equations to measure the magnesium concentration in perennial ryegrass herbage (Lolium perenne). A subset of 72 samples was selected on the basis of spectral variation from 400 samples grown in 1988-1989. Three alternative equations were chosen using stepwise multiple linear regression, with standard errors ranging from 0.4 to 0.3 g/kg DM with corresponding squared multiple correlation coefficients ( R2) of 0.68 to 0.82. The equations had 2, 4 and 4 wavelength terms respectively. When these equations were tested on an independent population of perennial ryegrass samples, a significant bias existed when using the 4 term equations but there was no bias when the 2 term equation was used. We conclude that NIR can be used to screen large numbers of perennial ryegrass plants for magnesium concentration. However, for the calibration equations to be used for the analysis of other populations equation performance must be monitored by comparing reference and NIR analyses on a small number of samples.