Abstract
BackgroundMembrane proteins such as cell adhesion molecules,receptors, transporters and ion channel proteins all haveessential roles in cell-growth, migration, and flow ofinformation, cell-cell and cell-protein communication.Membrane proteins are targets of biopharmaceuticalcompanies because they have diverse effects on the pro-gression of many diseases[1]. Ion channels are mem-brane proteins that play critical roles in a number ofcell functions including communication and neuromus-cular activity. Treatment of channelopathy diseases suchas cancer, cardiac arrhythmia, ataxia, paralysis, epilepsy,memory and learning loss, requires a broad understand-ing of ion channel function.Sialic acid is a criticalcharged glycan that affects the action potential of potas-sium channels which leads to changes in the neuronalsystem of organisms. In order to understand the effectof the sialylation on channel function, the presence ofthe sialic acid on the protein of interest should be stu-died. In this study, a novel method for the quantificationof sialic acid is described for a potassium channel mem-brane protein.Materials and methods
Highlights
Membrane proteins such as cell adhesion molecules, receptors, transporters and ion channel proteins all have essential roles in cell-growth, migration, and flow of information, cell-cell and cell-protein communication
After 15 days of antibiotic selection, an expression level of the gene of interest was evaluated by Western blot
The stable pool expression is an average of the varied expression levels of the protein of interest in cells
Summary
Membrane proteins such as cell adhesion molecules, receptors, transporters and ion channel proteins all have essential roles in cell-growth, migration, and flow of information, cell-cell and cell-protein communication. Ion channels are membrane proteins that play critical roles in a number of cell functions including communication and neuromuscular activity. Treatment of channelopathy diseases such as cancer, cardiac arrhythmia, ataxia, paralysis, epilepsy, memory and learning loss, requires a broad understanding of ion channel function. Sialic acid is a critical charged glycan that affects the action potential of potassium channels which leads to changes in the neuronal system of organisms. In order to understand the effect of the sialylation on channel function, the presence of the sialic acid on the protein of interest should be studied. A novel method for the quantification of sialic acid is described for a potassium channel membrane protein
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