Year-end Sale % OFF 
Abstract
A direct and nondestructive method for the quantitative determination of serum or plasma triglycerides has been developed. The lipid extract of 1 ml of serum is chromatographed on I-mm wet-thickness silicic acid layers prepared from a water–acetone slurry. Following elution, triglycerides are quantified by infrared spectrophotometry. For both synthetic and naturally occurring triglycerides, a nonlinear relationship of absorptivity as a function of concentration has been characterized. Triglyceride fatty acid chain length and degree of unsaturation have no significant effect on analytical results under the conditions described. Precision of the method is 2% and accuracy 4%. Recoveries average 96%. The method is sensitive to serum concentrations as low as 20 mg/100 ml. Triglyceride levels of a group of 51 healthy males, age 40 to 59, were determined. The mean postabsorptive triglyceride value was 93 mg/100 ml, with a standard deviation of 43 mg/100 ml. The system permits preservation of the triglyceride molecule intact for subsequent procedures such as characterization of the fatty acid moiety and substitution pattern.
Highlights
This paper describes the use of thin-layer silicic acid important role in lipid metabolism ( 1 4 )
I n addition, infrared methods have been used t o measure lipid fractions following their separation by means of silicic acid column chromatography [8]
The values obtained by analysis were compared with those calculated on the basis of the triglyceride concentration of the undiluted serum as measured by the thin-layer chromatography (TLC)-infrared method
Summary
Plates ( 5 ) ,has found wide application in lipid analysis. This procedure permits separation and excellent resolution of the major lipid classes. Lipids separated by this method have been quantified by densitometry after charring [6], or by colorimetry following elution of the lipid spot [7]. I n addition, infrared methods have been used t o measure lipid fractions following their separation by means of silicic acid column chromatography [8]. All solvents used were either of spectrophotometric quality or redistilled analytical reagent grade. The residue, if any, was dissolved in 0.5 ml carbon disulfide, and the infrared spectrum examined for interfering substances. The spectrum of each batch of carbon disulfide was obtained and compared to that supplied by the manu-.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
