Measurement of Serum IgG in Foals by Radial Immunodiffusion and Automated Turbidimetric Immunoassay

Abstract

Hypogammaglobulinemia as a result of failure of transfer of passive immunity (FTPI) is an important risk factor for infectious disease in neonatal foals. The current gold standard for determining serum immunoglobulin concentrations is radial immunodiffusion (RID). The purpose of this study was to compare immunoglobulin concentrations measured by RID with those determined by an automated turbidimetric immunoassay (TIA), which has a much shorter turnaround time. Immunoglobulin concentrations were measured by both RID and TIA in serum collected from 84 neonatal foals. Sixty‐seven foals had results within the linear range for both assays. Sensitivity and specificity of TIA for diagnosis of FTPI with IgG ≤ 800 mg/dL were 0.81 (95% CI 0.70‐0.88) and 0.86 (95% CI 0.76‐0.93) and with IgG ≤ 400 mg/dL were 0.63 (95% CI 0.35‐0.86) and 0.92 (95% CI 0.87‐0.95), respectively. A significant linear relationship was found between IgG concentrations determined by TIA and RID (TIA = 0.9511RID + 8.4354;R2= .59,P< .0001). The coefficients of variation for between‐run and within‐run precision for the TIA were 2.5 and 3%, respectively. Storage of samples from 10 foals at ‐20°C for 10–12 months resulted in a reduction in TIA‐measured serum IgG concentration of ‐17.6% (SD = 3.7%), indicating that long‐term storage of samples at ‐20°C should be avoided. The results of this study indicate that measurement of serum IgG by TIA can be used to evaluate foals for FTPI.