Measurement of serum glutathione peroxidase, catalase and superoxide dismutase concentration in patients with external anogenital warts before and after treatment with intralesional tuberculin purified protein derivative.

Abstract

External anogenital warts are considered the commonest viral sexually transmitted infection that has serious negative psychological effects on both males and females such as anxiety and marked stress. Aim of this work is to measure serum glutathione peroxidase (GSH-Px), catalase and superoxide dismutase enzyme level in patients with external anogenital warts before and after intralesional tuberculin purified protein derivative (PPD) injection. This study carried out 59 patients with anogenital warts recruited from the dermatology clinic and the andrology clinic, Suez Canal University hospital. Each patient was injected with 10 units (0.2ml) of PPD intralesionally in the mother or largest wart with 2weeks interval till complete resolution or maximum of six injections. Serum GSH-Px, catalase and superoxide dismutase were measured using ELISA before and after injection. Results of PPD injection showed that 69.5% of patients were responders while 30.5% were nonresponders. The mean level of serum GSH-Px, catalase and superoxide dismutase dropped significantly after injection with levels pre- and post-injection, 91.1ng/ml versus 52.6ng/ml, 88.5ng/ml versus 67.4ng/ml and 28.6ng/ml versus 21.8ng/ml respectively. Antioxidant enzyme levels decrease significantly after PPD injection. Serum GSH-Px and catalase were significantly related to PPD response.