Abstract
Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl- 3H]adenosyl- l-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl- 3H]adenosyl- l-methionine from [ methyl- 3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.
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