Abstract
The measurement of renin activity, concentration, and substrate in rat plasma has been performed by radioimmunoassay of the angiotensin I generated during incubation of plasma in vitro. The sensitivity of the method permits measurements of all three renin parameters in small plasma samples, allowing sequential determinations to be performed in individual animals. The specificity of the method has been demonstrated by the complete absence of renin in plasma from nephrectomized male rats, by the identical assay slopes of generated angiotensin and angiotensin I standards, and by the parallel changes in plasma renin activity (PRA) and renin concentration (PRC) measured by bioassay and immunoassay after furosemide administration and water deprivation. Also, radioimmunoassay determination of the pH optimum for renin activity, and of the correlations between PRA and PRC, were identical with those obtained by bioassay. The absolute values for renin substrate were similar to those measured by bioassay. However, th...
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