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Abstract
Accurate measurement of pyruvate is particularly important in the detection of mitochondrial enzyme disorders. We describe a simple high-performance liquid chromatography assay for pyruvate with derivatization and fluorescence detection. Between-run variation is below 4·5% and the method is linear between 1 and 1200μmol/L. The mean recovery is 101%. After sample pretreatment, the calculated pyruvate concentration proved to be stable for 15 days when samples were kept at −20°C.
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