Abstract

The rate of change of pH caused by the hydrolysis of urea was measured for urease solutions of 18 different concentrations. Concentrations were converted into an activity, A (measured in IU/cm 3), by using a titrimetric method to assay the urease sample. For activities in the range 0.6–38 IU/cm 3, A was related to the initial rate of change of pH, ( dpH/ dt) 0 , (measured in s −1) by the empirical relationship: A = 549( dpH/ dt) 0 − 1423( dpH/ dt) 0 2 . Values of ( dpH/ dt) 0 were sensitive to changes in the urease activity of about 0.6 IU/cm 3.

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