Abstract
A micromethod was developed for the measurement of UDP-glucuronic acid present in samples as small as 60 mg of liver, kidney, small intestine, heart, or muscle. It utilizes the highly efficient tyramine-glucuronidating system, catalyzed by UDP-glucuronyltransferase of guinea pig liver. The reaction is specific for UDP-glucuronic acid; UDP derivatives of glucose, galactose, N-acetylglucosamine, and xylose were inactive as conjugating agents. The concentration of UDP-glucuronic acid, expressed as micromoles per 100 g wet wt of the following tissues of guinea pig, rat, and mouse are, respectively: liver, 56.7, 15.9, and 16.6; kidney, 5.9, 4.0, and 8.8; small intestine, 6.3, 3.2, and 12.3; brain, 1.66, 1.08, and 1.68. The corresponding values for rat heart and muscle were 0.96 and 0.35.
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