Measurement of multispectral scattering properties in mouse brain tissue

Eunjung Min,Gabriel Popescu,Woonggyu Jung,Sungbea Ban,Sung Chul Bae,Catherine Best-Popescu,Yanyan Wang

doi:10.1364/boe.8.001763

Abstract

We present the scattering properties of mouse brain using multispectral diffraction phase microscopy. Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm. The regional analysis was performed for both the myeloarchitecture and cytoarchitecture of the brain tissue. Our results clearly evaluate the multispectral scattering properties in the olfactory bulb and corpus callosum. The scattering coefficient measured in the corpus callosum is about four times higher than in the olfactory bulb. It also indicates that it is feasible to realize the quantitative phase microscope in near infrared region for thick brain tissue imaging.

Highlights

The human brain contains roughly 1012 neurons, which together result in an estimated 1015 connections [1]
We present the scattering properties of mouse brain using multispectral diffraction phase microscopy
Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm

Summary

Introduction

The human brain contains roughly 1012 neurons, which together result in an estimated 1015 connections [1]. Recovering a complete picture of all the neuronal connections in the brain, the Connectome, represents a formidable challenge for the imaging field. Routine clinical tools such as MRI and PET have the ability to image the brain in vivo [2, 3]. On the other hand, can extract information with submicron resolution The limitation in this case is penetration depth, which is capped by the transport mean free path of the tissue. Quantitative phase imaging (QPI) [9] has been developed in response to the need for label-free, intrinsic contrast imaging, as well as quantitative information about the tissue structure. The results show quantitatively the boost in tissue penetration provided by infrared vs. visible light

Sample preparation

Imaging

Result

Discussion and conclusions