Measurement of microcystin -LR in water samples using improved HPLC method.

Hamid Reza Shamsollahi,Shahrokh Nazmara,Ramin Nabizadeh,Amir Hossein Mahvi,Mahmood Alimohammadi

doi:10.5539/gjhs.v7n2p66

Hamid Reza Shamsollahi, Shahrokh Nazmara + Show 3 more

Open Access

https://doi.org/10.5539/gjhs.v7n2p66

Copy DOI

Abstract

Microcystins are a group of toxic compounds produced by freshwater cyanobacteria and cause diseases. World Health Organization has recommended a concentration of 1 µg/l for Microcystin-LR (MC-LR) in potable water as guideline value. The high performance liquid chromatography (HPLC) followed by C18 analytical column and ultra violet detector for detection of MC-LR. In this regard, 5 different concentrations of MC-LR solutions were injected into HPLC. MC-LR was detected in 5.33 minute retention time and Calibration curve was achieved with R2=0.988. Detection limit for this method was obtained by using acetonitrile solutions (32% and 55%) as a gradient run and a high silanol activity column equal to 0.02 µg/mL. Despite no acidic organic modifier being used in the mixture of solvents, the sensitivity of this method was appropriate for detection of MC-LR. Because of short retention time, reduction in number of solvents and high resolution and suitable sensitivity, this method is affordable and is fast for detection and determination of MC-LR in potable water.

Highlights

Safe drinking water is very important as a matter of health point of view in a community
5 different concentrations of MC-LR solutions were injected into high performance liquid chromatography (HPLC)
MC-LR is a hydrophilic variant of MCs, so polar solvents are suitable for elution of MC-LR molecules in reversed phase chromatography

Summary

Introduction

Safe drinking water is very important as a matter of health point of view in a community. Microcystins (MCs) are a group of toxic compounds being produced by freshwater cyanobacteria, such as Anabena, Nostoc, microsistis and Oscillatoria. The X and Z types are two variable L-Amino acid in position 2 and 4 of cyclic structure (Dawson, 1998, Edwards, Christine, Linda, & Lawton, 2010; Lawton, Linda, & Christine Edwards, 2001; Sangolkar, Lalita et al, 2006). Microcystin-LR (MC-LR) is one of the highest toxic MCs that contain Lucien (L) and Arginine (R). Exposure to high levels of MCs can cause hepatocyte necrosis, hemorrhage, and death. Long term exposure to low levels of toxin causes tumor promotion in the body (Lawton, Linda, & Christine Edwards, 2001; Sangolkar, Lalita et al, 2006)

Objectives

Methods

Findings

Discussion

Conclusion