Abstract
The Abbott Bichromatic Analyser (ABA-100), an instrument currently in widespread use in clinical chemistry laboratories, was used to measure concentrations of metabolic intermediates in neutralized perchloric extracts from perfused rat hearts. Results were similar to those obtained by others using manual methods. Excellent reproducibility was achieved with metabolites present in higher concentrations (upward of 50 μ m). Lower levels of substances could be measured with precision (coefficient of variation <5%) down to extract concentrations of about 10 μ m. The ABA-100, therefore, offers the advantages of speed and economy over existing methods, combined with a high degree of reproducibility. It is concluded that this type of instrument has considerable potential as a research tool.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
