Measurement of gastric acid secretion in isolated gastric mucosa of the rat: effects of secretagogues and inhibitors including cyclic adenosine monophosphate related agents and an H2-receptor antagonist.

Abstract

An improved method was described for the measurement of gastric acid secretion of isolated rat gastric mucosa. With this method, dose-dependent acid secretory responses were obtained to secretagogues such as histamine, bethanechol, tetragastrin and dibutyryl cyclic adenosine monophophate (AMP). However, the maximum response to tetragastrin was always smaller than that to the other secretagogues in this preparation. Inhibitory effects on acid secretion of some antisecretory agents were also examined. Antagonistic interactions of atropine against bethanechol, and of metiamide against histamine were clearly confirmed. On the other hand, inhibitions of metiamide on bethanechol and of atropine on tetragastrin or histamine were found rather weak in contrast to the results obtained with other methods. Strong stimulatory effect of theophylline and absence of the influence of dibutyryl cyclic GMP were shown in our preparation. The actions of miscellaneous agents including prostaglandin E1, imidazole and metabolic inhibitors were also described.