Abstract
Multiple frequency bioelectrical impedance analysis (MFBIA) was used to measure whole body impedance of rats between frequencies from 1 kHz to 100 kHz. The data were analysed, with reference to an equivalent electrical circuit for biological tissue, to estimate total body water (TBW) and extracellular water (ECW). TBW and ECW were independently determined by isotope dilution and desiccation. Correlation between MFBIA measurements and these alternative techniques were r=0.984 (SEE=6.5%) and r=0.995 (SEE=3.2%) for TBW and ECW respectively. These results establish the validity, accuracy and precision of MFBIA for the assessment of both TBW and ECW.
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