Abstract
AbstractThe inhibitor of ornithine decarboxylase, DL‐α‐difluoromethylornithine (DFMO), shows considerable potential in the chemotherapeutic control of lethal protozoal infections. It is also valuable in studies of the metabolism of polyamines. This paper describes a method for the accurate assay of the concentration of this material in aqueous tissue extracts by determining the degree to which a yeast ornithine decarboxylase, expressed on a high‐copy‐number Escherichia coli plasmid, is inhibited. By relating the degree of inhibition to that achieved with standard amounts of DFMO, the concentration present in unknown samples may be determined. The method has been applied to an examination of the conversion by transformed root cultures of Datura stramonium of DL‐α‐difluoromethylarginine to DFMO.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
