Abstract

Simultaneous dialysis of 2 diluted plasma samples in the same system at 37°C is an acceptable method for measurement of the relative amounts of corticosteroid binding protein present. Dissociation of the steroid-protein complex in undiluted samples is slow even at 37°. The kinetics of binding do not permit attainment of equilibrium at 4°, even when the plasma is diluted. The differences noted by previous investigators in comparison of pregnant and non-pregnant samples are explainable by this incomplete equilibrium.

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