Abstract
Total dissolved inorganic carbon (SigmaCO(2)) and aqueous carbon dioxide (H(2)CO(3) (*)) in nutrient solutions may be measured by the injection of small gas or liquid samples (1 microliter to 8 milliliters) into a gas stripping column connected in-line with an infrared gas analyzer. The measurement of SigmaCO(2) in solution requires sample acidification, while H(2)CO(3) (*) and gaseous CO(2) are measured without the addition of lactic acid. The standard curve for SigmaCO(2) was linear up to 300 nanomoles CO(2). Maximum sensitivity was approximately 300 picomoles. Measurements of H(2)CO(3) (*) were independent of pH. Consequently, SigmaCO(2) and H(2)CO(3) (*) could be used to calculate the pH, HCO(3) (-), and CO(3) (2-) values of nutrient solutions. Injection and complete analyses required from 0.8 to 2 minutes.
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