Measurement of Ca 2+ currents in intact slow skeletal muscle fibers of the frog by the three-microelectrode technique

Abstract

The recording of currents passing through calcium channels in intact skeletal muscle fibers presents several difficulties. However, use of the three-microelectrode voltage-clamp technique at the end of the fiber provided us with a good approximation of current values in such fibers. Using this technique, we were able to measure the calcium-channel current in slow skeletal muscle fibers of the frog ( Rana pipiens) and to quantify the effects of denervation on this current.