Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes.

Abstract

During the process of differentiation from induced pluripotent stem cells (iPSCs) to hepatocyte-like cells it is crucial to monitor the functionality of cells, in order to avoid an overestimation of the level of maturation. To this end, we propose bile acid profiling as a novel approach which is useful in determining the maturation of hepatocyte-like cells. The main advantages of the method are the simplicity and rapidity of test (i.e., single-step sample preparation followed by 3.5-min analysis), as well as the possibility to localize possible enzyme deficiencies by quantifying the accumulation of specific intermediates involved in the synthetic pathways.