Abstract
In the present study, a sensitive label–free electrochemical aptasensor is introduced to measure aflatoxin M1 (AFM1) by using platinum nanoparticles (PtNPs) decorated on a glassy carbon electrode (GCE) modified with Fe–based metal–organic frameworks, MIL–101(Fe). The MIL–101(Fe) and the PtNP/MIL–101(Fe) are synthesized and characterized by Fourier transform infrared spectroscopy, X–ray diffraction, UV–Visible spectroscopy, and field–emission scanning electron microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy (EIS) are done to monitor the fabrication processes of the aptasensor. In optimum conditions, the linear calibration range of 1.0 × 10−2 to 80.0 ng mL−1 and the detection limit of 2.0 × 10−3 ng mL−1 are obtained to measure AFM1 concentration using the EIS method. Finally, the fabricated aptasensor is successfully applied to measure AFM1 concentration in powder and pasteurized milk samples.
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