Abstract
Chang1'2 has recently reported on the nutritional and cultural requirements of Leptospira icterohaemorrhagiae. Although he showed that this organism was an obligate aerobe, Chang was unable to demonstrate any oxygen uptake in the Warburg respirometer by 18,000,000 leptospiras over a period of 6 hours. In our experience in measuring the respiration of trypanosomes, we would expect to obtain only a very small oxygen uptake from this number of trypanosomes. Since the trypanosome is many times larger than the spirochete, it seemed to us that the failure of Chang to obtain a measurable respiration was due to insufficient material in the res-
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