Measurement of 8-OHdG in DNA by HPLC/ECD: the importance of DNA purity.

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BioTechniquesVol. 20, No. 1 BenchmarksOpen AccessMeasurement of 8-OHdG in DNA by HPLC/ECD: The Importance of DNA PurityGeorge M. Laws & Stephen P. AdamsGeorge M. LawsMerck Research Laboratories, West Point, PA, USASearch for more papers by this author & Stephen P. Adams*Address correspondence to Stephen P. Adams, Genetic and Cellular Toxicology, WP 45-307, Merck Research Laboratories, West Point, PA 19486, USA. Internet: E-mail Address: stephen_adams@merck.comMerck Research Laboratories, West Point, PA, USASearch for more papers by this authorPublished Online:2 Aug 2018https://doi.org/10.2144/96201bm06AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail FiguresReferencesRelatedDetailsCited ByAssessment of two DNA extraction kits for profiling poultry respiratory microbiota from multiple sample types6 January 2021 | PLOS ONE, Vol. 16, No. 1GSTP1 c.313A>G polymorphism in Russian and Polish athletesPhysiological Genomics, Vol. 49, No. 3Paper-based solid-phase microextraction for analysis of 8-hydroxy-2’-deoxyguanosine in urine sample by CE-LIF28 November 2016 | ELECTROPHORESIS, Vol. 38, No. 3-4Mitochondria-targeted catalase reduces abnormal APP processing, amyloid production and BACE1 in a mouse model of Alzheimer's disease: implications for neuroprotection and lifespan extension5 April 2012 | Human Molecular Genetics, Vol. 21, No. 13Aging and amyloid beta-induced oxidative DNA damage and mitochondrial dysfunction in Alzheimer's disease: Implications for early intervention and therapeuticsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol. 1812, No. 11Product Specifications and Quality Control18 July 2011OPTIMIZATION OF DNA EXTRACTION FROM BROWN ALGAE (PHAEOPHYCEAE) BASED ON A COMMERCIAL KIT11 April 2010 | Journal of Phycology, Vol. 46, No. 3Platinum(II)−Dendrimer Conjugates: Synthesis and Investigations on Cytotoxicity, Cellular Distribution, Platinum Release, DNA, and Protein Binding27 January 2010 | Bioconjugate Chemistry, Vol. 21, No. 2Hepatitis B viral X protein alters the biological features and expressions of DNA repair enzymes in LO2 cellsLiver International, Vol. 30, No. 2Detection of hybrids between wild boars (Sus scrofa scrofa) and domestic pigs (Sus scrofa f. domestica) in Greece, using the PCR-RFLP method on melanocortin-1 receptor (MC1R) mutationsMammalian Biology, Vol. 75, No. 1Chloroacetonitrile induces oxidative stress and apoptosis in mouse fetal liverToxicology Letters, Vol. 190, No. 2Effects of Chemical Carcinogens and Physicochemical Factors on the UV Spectrophotometric Determination of DNAJournal of Toxicology and Environmental Health, Part A, Vol. 68, No. 23-24DNA damage in digestive gland and mantle tissue of the mussel Perna pernaComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Vol. 135, No. 3Inhibition of vancomycin-induced nephrotoxicity by targeting superoxide dismutase to renal proximal tubule cells in the rat19 July 2013 | Redox Report, Vol. 7, No. 5Oxidative stress in the mussel Mytella guyanensis from polluted mangroves on Santa Catarina Island, BrazilMarine Pollution Bulletin, Vol. 44, No. 9Effects of diethylmaleate on DNA damage and repair in the mouse brainFree Radical Biology and Medicine, Vol. 33, No. 2Genotoxicity of naturally occurring indole compounds: correlation between covalent DNA binding and other genotoxicity tests25 July 2002 | Environmental and Molecular Mutagenesis, Vol. 40, No. 1Induction of chromosome aberrations in vitro by phenolphthalein: mechanistic studiesMutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 457, No. 1-2Increased formation of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 and COMT genotypesCancer Letters, Vol. 151, No. 1Chromatographic determination of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cellular DNA: A validation study7 July 2009 | Free Radical Research, Vol. 33, No. 3Oxidative DNA damage in the aging mouse brain1 January 2001 | Movement Disorders, Vol. 14, No. 6Oxidative damage to mitochondrial DNA and its relationship to diabetic complicationsDiabetes Research and Clinical Practice, Vol. 45, No. 2-3Attenuation of age-dependent oxidative damage to DNA and protein in brainstem of Tg Cu/Zn SOD miceNeurobiology of Aging, Vol. 19, No. 4Detection of DNA damage induced by human carcinogens in acellular assays: Potential application for determining genotoxic mechanismsMutation Research/Genetic Toxicology, Vol. 368, No. 3-4System Issues: Mutagenic response of the endogenoushprt gene andlacl transgene in benzo[a]pyrene-treated Big Blue™ B6C3F1 miceEnvironmental and Molecular Mutagenesis, Vol. 28, No. 4 Vol. 20, No. 1 Follow us on social media for the latest updates Metrics Downloaded 1,761 times History Published online 2 August 2018 Published in print January 1996 Information© 2018 Author(s)PDF download