Measurement and modelling of ABA signalling in potato ( Solanum tuberosum L.) during partial root-zone drying

Abstract

The objective of this study was to develop a simple mechanistic model to predict the magnitude of ABA signalling ([X-ABA]) of potatoes ( Solanum tuberosum L.) exposed to partial root-zone drying (PRD). Potatoes were grown in pots in a glasshouse with the roots split equally between two soil columns. At tuber initiation stage, plants were subjected to three irrigation treatments: (1) both soil columns were fully irrigated (FI) daily to a volumetric soil water content ( θ) of 18.0%; (2) PRD, in which one soil column was irrigated daily to 18.0% while the other was allowed to dry, and the irrigation was shifted between columns when the θ of the drying soil column had decreased to 7–8%; (3) non-irrigation (NI), where irrigation was withheld after onset of treatments and lasted for 5 days until θ had decreased to 7%. In the PRD plants, the fraction of soil water extraction (FSWE) by the dry roots declined exponentially with declining soil water potential ( Ψ soil-dry); however, after shifting of irrigation, the previously dried roots immediately recovered the full capacity of water uptake. During the first PRD drying cycle, FI plants had the highest stomatal conductance ( g s), and followed by PRD plants and NI plants had the lowest g s. Photosynthesis ( A) was similar for FI and PRD plants, and was significantly lower for the NI plants only on 3–4 days after treatment. In the NI plants, a linear relationship between Ψ soil and [X-ABA] was obtained. Based on these relationships, a simple model predicting [X-ABA] in the PRD plants ([X-ABA] PRD) was developed. Assuming that a constant [X-ABA] of 115 nM (similar to that found in the FI plants) originated from the wet roots; the simulation results indicated that irrigation should be shifted between the two sides when Ψ soil-dry had decreased to ∼−80 kPa, and [X-ABA] PRD had reached a peak of ca. 150 nM. However, the [X-ABA] PRD predicted by the model was significantly lower than the measured value; whilst a simple average of [X-ABA] from the wet and the dry soil columns based on the [X-ABA]– Ψ soil relationship better predicted [X-ABA] PRD.