MDM2 gene a mplification in s kin c ancer p atients

Abstract

Skin cancer: Skin cancer is the uncontrolled proliferation of abnormal skin cells; skin cancer is classified into non-melanoma (squamous cell carcinoma and basal cell carcinoma) and melanoma. Approximately, 90% of skin cancers are caused by ultraviolet radiation, because of ultraviolet radiation emits to damage of skin cells DNA resulting in genetic mutations and formation of skin cancer.
 Materials and methods: This study evaluated paraffin blocks from 17 specimen randomly selected from patients with skin cancer, and 6 specimens from patients non cancer patients as control group for comparison .This study was carried out in Laboratories of College of Science, Wasit University and AL Zahraa and ALKarama Teaching Hospitals, from October 2014 to April 2015. In this study MDM2( murine double minute 2) gene amplification was assessed by chromogenic in situ hybridization (CISH) technique.
 Results: This study showed that there was no significant difference between skin cancer patients and control group in relation to MDM2 amplification (p>0.05), but presence of highly significant difference between patients of skin cancer and control group in relation to amplification intensity of MDM2 (p<0.05). Moreover, and according to histological type, our finding found significant difference between the types of skin cancer in relation to MDM2 amplification (p<0.05). Additionally, there were significant differences between patients of basal cell carcinoma and squamous cell carcinoma and control group in relation to MDM2 amplification(p<0.05).
 Conclusion: We conclude that MDM2 amplification was high in types of skin cancer (BCC and SCC) because of MDM2 amplification in keratocytes of the epidermis may be associated with entry of these cells into the differentiation program.