MDM2 AND p53 IN CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA: Higher Expression in Childhood Leukemias with Poor Prognosis Compared to Long-Term Survivors

Abstract

In previous studies the authors have found increased expression of p53 and MDM2 proteins in leukemic cells in a majority of children eligible for bone marrow transplantation (BMT) due to relapse orprognostically unfavorable features. In this study theimmunohistochemical expression of p53, MDM2, and p21 Cip1 was investigatedin bone marrow samples from the time of diagnosis in 30 children with acute lymphoblastic leukemia (ALL) surviving disease-free at least 5 years. This group was compared with 15 advanced ALL patients, admitted for BMT. In 7 of the BMT patients original diagnostic marrow samples were also available for analysis. Four out of 30 ALL patients in the relapse-free group expressed p53 in theoriginal leukemic cell population, while 8/15 advanced ALL patients did before BMT (p = .014 ). Four out of 30 cases in the relapse-free group expressed MDM2, while 10/15 in the BMT group did (p = .0011 ). In retrospect, MDM2 over expression at the time of diagnosis was also more common (p = .0098 ) in the BMT group as well as p53 over expression (p = .054 ) compared to nonrelapsed patients.