Abstract
We detected the mcr-1 gene in 21 (14.8%) Salmonella isolates from pigs at slaughter; 19 were serovar Typhimurium sequence type 34. The gene was located on IncHI2-like plasmids that also harbored IncF replicons and lacked a conjugative transfer region. These findings highlight the need to prevent further spread of colistin resistance in animals and humans.
Highlights
We detected the mcr-1 gene in 21 (14.8%) Salmonella isolates from pigs at slaughter; 19 were serovar Typhimurium sequence type 34
We used Hiseq Technology (Illumina, San Diego, CA, USA) to sequence plasmid DNA purified from transformants of Salmonella Heidelberg SH36 and Salmonella London Z4P319S and genomic DNA extracted from the original isolate (Salmonella Typhimurium SH138, which was selected as a representative ST34 strain)
We found that spread of mcr-1 in pigs at slaughter in China was associated with clonal dissemination of Salmonella Typhimurium ST34
Summary
We detected the mcr-1 gene in 21 (14.8%) Salmonella isolates from pigs at slaughter; 19 were serovar Typhimurium sequence type 34. We determined MICs for 12 antimicrobial drugs for all mcr-1–positive isolates by using agar dilution methods or a broth microdilution method. Plasmid-mediated resistance genes floR (florfenicol resistance) and oqxAB (olaquindox and ciprofloxacin resistance) were detected by using PCR.
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