Abstract
The aim of this study was to examine the effects of MCI-154, a new positive inotropic agent with vasodilating properties, on the Ca 2+-activated K + channel (K Ca channel) of vascular smooth muscle cells. Cultured smooth muscle cells from a porcine coronary artery were studied using the patch-clamp technique. Extracellular application of 100 μM MCI-154 activated the K Ca channel in intact cell-attached patch configurations. In excised inside-out patch configurations, application of 100μM MCI-154 to the cytosolic side activated the K Ca channel directly, suggesting that the Ca 2+ sensitivity of the K Ca channel itself is modulated. Though extracellular application of 100 μM amrinone, a phosphodiesterase inhibitor, activated the K Ca channel in the cell-attached patch configurations, application of 100 μm amrinone to the cytosolic side could not activate the K Ca channel in inside-out patch configurations. These results indicate that different from amrinone, MCI-154 can modulate Ca 2+ sensitivity of the K Ca channel in vascular smooth muscle cells.
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