Abstract
Materials and Methods: In order to follow mutational and epigenetic status of the selected genes eighteen formalin fixed paraffin embedded (FFPE) primary and recurrent tumor tissue samples were selected. Eleven samples had pure mixoid histology, one was mixoid with <5% of round cell component and the others were PLS. Results: Immunohistochemical analysis revealed that p53 protein was overexpressed in 3/12 MLPS (25%) and 6/6 PLS (100%). Mutational analysis showed that two out of eleven MLPS (2/11, 18.2%) and two out of six PLS (2/6, 33.3%) contained mutated p53 gene. However, the frequencies of the p14ARF gene methylation were 83.3% (10/12) and 50% (3/6) in mixoid and pleomorphic group, respectively. Overall, 15 out of 18 (83.3%) samples had either p53 mutation or methylated p14ARF. Conclusions: The results from the current study suggest paramount importance of the p14ARF gene methylation in the pathogenesis and progression of mixoid and to a lesser extent pleomorphic liposarcoma.
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