Abstract
BackgroundAsthmatic nasal polyps primarily exhibit eosinophilic infiltration. However, the identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. Thus, we thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea.MethodsTissues from uncinate process (UP) were obtained from controls (n = 18) and CRS without nasal polyps (CRSsNP, n = 45). Nasal polyps (NP) and UP were obtained from CRS with nasal polyps (CRSwNP, n = 56). The innate immune cells was evaluated by immunohistochemistry such as, eosinophil major basic protein (MBP), tryptase, CD68, CD163, CD11c, 2D7, human neutrophil elastase (HNE) and its distribution was analyzed according to clinical parameters.ResultsIn comparisons between UP from each group, CRSwNP had a higher number of MPB+, CD68+, and CD11c+ cells relative to CRSsNP. Comparisons between UP and NP from CRSwNP indicated that NP have a higher infiltrate of MBP+, CD163+, CD11c+, 2D7+ and HNE+ cells, whereas fewer CD68+ cells were found in NP. In addition, MBP+ and CD11c+ cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP. Moreover, in UP from CRSwNP, the number of MBP+ and CD11c+ cells positively correlated with CT scores. In the analysis of CRSwNP phenotype, allergic eosinophilic polyps had a higher number of MBP+, tryptase+, CD11c+, 2D7+ cells than others, whereas allergic non-eosinophilic polyps showed mainly infiltration of HNE+ and 2D7+ cells.ConclusionsThe infiltration of MBP+ and CD11c+ innate immune cells show a significant association with phenotype and disease extent of CRS and allergic status also may influences cellular phenotype in non-asthmatic CRSwNP in Korea.
Highlights
Chronic rhinosinusitis (CRS) is one of the most common chronic inflammatory diseases of the nasal and paranasal sinuses
Examination of the cell populations in uncinate process (UP) and Nasal polyps (NP) tissues from the CRS with nasal polyps (CRSwNP) group showed that greater numbers of major basic protein (MBP)+ cells, CD11c+ cells, 2D7+ cells, and human neutrophil elastase (HNE)+ cells were present in NP tissues, whereas the number of CD68+ cells were decreased in NP tissues
The MBP+ and CD11c+ cells were increased from UP of CRSsNP, to UP of CRSwNP, and to NP of CRSwNP (Fig. 2A and 2E)
Summary
Chronic rhinosinusitis (CRS) is one of the most common chronic inflammatory diseases of the nasal and paranasal sinuses. CRS is divided into two types: CRS with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP) [2,3] In the past several decades, numerous studies have been performed to investigate nasal polypogenesis in patients from Western countries. These studies suggest that CRSwNP in Western countries is characterized by a Th2-based immune response with abundant eosinophilic infiltration, high levels of Interleukin (IL-5), and low levels of TGF-b. The identities of the immune cells that infiltrate non-asthmatic nasal polyps remain unclear. We thought to investigate the distribution of innate immune cells and its clinical relevance in non-asthmatic chronic rhinosinusitis (CRS) in Korea
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