Maximizing unsaturated fatty acids production by using sugarcane agroindustry wastes in cultivation of Desmodesmus sp. in a flat plate photobioreactor

Abstract

Microalgae lipid accumulation can be accomplished by different strategies rather than naturally reaching the stationary phase. Many studies employ nitrogen (N) depletion to improve lipid production; however, this approach might not be a suitable alternative when growth in wastewater is attempted. Agro-industry effluents in particular can have high concentrations of N, so nutrient removal is also required. This study evaluated two possibilities of achieving stress conditions in Desmodesmus sp. cultivation: light intensity and CO2 concentration. The culture medium also included liquid and solid residues from the sugarcane agro-industry: vinasse and a biofertilizer produced from bagasse biochar. Optimization of growth in a flat plate photobioreactor was conducted by combining a two-level factorial design and simplex methodology. Both the highest biomass and polyunsaturated fatty acid productivities (150.2 and 21.4 mg L−1 day−1, respectively) were achieved near the central points (5% CO2 in air and 1000 μmol m−2 s−1 light intensity). These results show the possibility of microalgae growth in a sustainable medium coupled with high-value lipid production, e.g., omegas-3, − 6, and − 9.