Maturational differences in the isolated isovolumic rabbit heart.

Abstract

To determine maturational differences in myocardial pressure work, oxygen consumption, and stability, we evaluated an isolated retrogradely perfused isovolumic rabbit heart model in newborns (0-6 days of age, mean 3.3 +/- 1.9 days), immatures (4-6 wk) and adults (5-7 mo). To reduce ischemic time and cardiac trauma, the hearts were isolated and instrumented within the chest cavity. By design, there were no significant age-related differences in coronary flow per gram, heart rate, or resting pressure. Left ventricular developed pressure was similar at all ages, but myocardial oxygen consumption was much lower in newborns and immatures than in adults. Coronary resistance was lower in the younger hearts. There were no significant age-related differences in contractility, left ventricular stiffness, or myocardial pH. All hearts were stable for 120 min, but thereafter immatures and adults deteriorated more rapidly than newborns. The study demonstrates that this model is technically suitable for the study of age-related differences in cardiac physiology. Furthermore, newborn and immature hearts are able to more efficiently use oxygen to develop left ventricular pressures, possibly due to a lower wall tension.