Maturational Changes in Brain Contents of Salmon GnRH in Rainbow Trout as Measured by a Newly Developed Time-Resolved Fluoroimmunoassay

Abstract

A newly developed time-resolved fluoroimmunoassay (TR-FIA) for salmon gonadotropin-releasing hormone (sGnRH) was applied to investigate changes in sGnRH content in discrete brain areas at three different gonadal stages in the rainbow trout, Oncorhynchus mykiss. The sensitivity (6.8 pg/well), specificity, intraassay coefficients of variation (<7.4%), and interassay coefficients of variation (<10.3%) of the assay system were almost the same as those for the radioimmunoassay. Displacement curves of serially diluted brain extracts of nine teleost fish (freshwater fish and seawater fish) including rainbow trout paralleled that of the sGnRH standard, indicating that the sGnRH TR-FIA is widely applicable to the measurement of the brain sGnRH contents of various fishes. The sGnRH content in female hypothalamus decreased during final gonad maturation, whereas the sGnRH levels in pituitary were highest at the time of spermiating in males or ovulating in females, decreasing significantly thereafter. In contrast, there were no changes in the sGnRH contents of olfactory bulbs, telencephalon, optic tectum + thalamus, and cerebellum + medulla oblongata during final maturation, except for olfactory bulbs of males. Changes in sGnRH contents in the hypothalamus and the pituitary indicate that sGnRH is involved in final maturation (ovulation or spermiation) in the rainbow trout.