Maturation of dendritic cells induced by nano-liposomes containing imiquimod

Abstract

Background: Imiquimod (IMQ), a small-molecule immune response modifier of the imidazoquinoline family, has been shown to activate functional maturation of dendritic cells (DCs) through the Toll-like receptor (TLR)7- signaling pathway. IMQ has the potential to develop into a novel vaccine adjuvant. Currently, a topical cream of IMQ has been approved for the treatment of genital warts, superficial basal cell carcinoma and actinic keratosis. Objective: To develop nano-liposomes with encapsulated IMQ as a new vaccine adjuvant. Methods: Two types of nano-lipid particles containing IMQ which differed in their surface charge (i.e. positively and negatively charged liposomes) were prepared and characterized. Murine bone marrow derived DCs were cultured and treated with liposomal formulations. The surface marker expression of DCs was then determined by flow cytometry. Results: Both liposomes with IMQ encapsulated enhanced DCs maturation as determined by the surface marker expression of DCs (CD80, CD86, and MHC class II). Cationic liposomes induced high levels of surface marker expression of DCs in similar levels as those induced by lipopolysaccharides (LPS). Conclusion: The novel IMQ formulation, nano-liposome, has the benefits of simplicity and it enhances the adjuvant effect of IMQ.