Abstract
P. sterna oocytes cannot be fertilized in vitro unless they are obtained by inducing spawning. The present work was undertaken to develop a simple method to induce germinal vesicle breakdown that would enable the oocytes to be fertilized without prior spawning. We found that Pteria sterna oocytes removed from the ovaries are induced to undergo germinal vesicle breakdown (GVBD) by 10 mM NH4Cl in seawater, and proceed to metaphase I of meiosis, at which stage they can be fertilized and will develop at least as far as "D" larvae. On the other hand, ovarian oocytes exposed to 2 × 10–5 M serotonin in seawater remained in prophase and did not undergo GVBD. Ammonium chloride treatment provides a simple, inexpensive procedure that can be useful in studies of fertilization and development, and for attempts to repopulate this exploited species.
Highlights
P. sterna es un bivalvo de interes comercial de la costa norte del Pacifico de Baja California (B.C.), Mexico
We found that Pteria sterna oocytes removed from the ovaries are induced to undergo germinal vesicle breakdown (GVBD) by 10 mM NH4CI in seawater, and proceed to metaphase I of meiosis, at which stage they can be fertilized and will develop at least as far as "D" larvae
After 5 h incubation with the drug, the percentage of oocytes with GVBD (4%) was the same as in control oocytes incubated without serotonin (3%)
Summary
P. sterna es un bivalvo de interes comercial de la costa norte del Pacifico de Baja California (B.C.), Mexico. Este trabajo pretende contribuir al estudio de la fecundaci6n in vitro de los ovocitos de dicho organismo (especificamente en cuanto a la inducci6n ala maduraci6n), ya que la informacion acerca de su biologia reproductiva es escasa, y con el prop6sito de contribuir en intentos de repoblaci6n de las zonas afectadas por su sobreexplotaci6n. En la mayoria de las especies de bivalvos, los ovocitos extraidos de las g6nadas que se encuentran en profase I, no son fertilizables excepto en los casos de Crassostrea gigas (Osanai, 1985) y Spisula solidissima (Allen, 1953). P. sterna is a bivalve of commercial interest on the north coast of Baja California (B.C.), Mexico. The present study was undertaken to develop a method for in vitro fertilization (especially the induction of oocyte maturation), which could be used to help in repopulating areas affected by overexploitation
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