Matrix-assisted laser desorption/ionization mass spectrometry using porous silicon and silica gel as matrix

Abstract

Porous silicon powder and silica gel particles have been applied as inorganic matrices for the analysis of small molecules in matrix-assisted laser desorption/ionization mass spectrometry (maldi-tofms). in contrast to conventional maldi-tofms, the signal interference of low-molecular analytes by the matrix has been eliminated. almost no fragmentations of the analytes were observed. effects of various factors, such as the particle and pore size, the suspending solution, and sample preparation procedures, on the intensity of mass spectra have been investigated. the pore structure of the inorganic matrix and penetration of the analytes into the pores must be optimized for effective desorption and ionization of the analytes. matrices (dhb and hcca) were covalently bound to silica gel for improvement of spectrum intensity. copyright (c) 2001 john wiley & sons, ltd.