Abstract
A method for the extraction and liquid chromatographic determination of five benzimidazole anthelmintics (thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole) in pork muscle tissue is presented. Blank or benzimidazole-fortified pork muscle tissue samples were blended with octadecylsilyl (C 18, 18% load, endcapped) derivatized silica packing material. A column made from this tissue/C 18 blend was washed with hexane then the benzimidazoles were eluted with acetonitrile. The acetonitrile eluate was purified by passing it through an activated alumina column. The purified eluates contained the benzimidazoles and were free of interferences as determined by liquid chromatography on ODS columns. The standard curves of individual benzimidazoles isolated from fortified samples, utilizing internal standards, were linear with average relative percentage recoveries of 86, 92, 85, 93, and 98% for thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole, respectively, over the concentration range of 100 to 3200 ng benzimidazole/g tissue. The interassay variabilities ranged from 4 to 7% and intraassay variabilities ranged from 2 to 3%.
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