Matrix Sensitivity and Interpretation for Precise Molecular Weight Analysis of Chitosan Hydrolysates

Abstract

The biological activity of chitosan differs depending on the molecular weight and degree of deacetylation, so more precise molecular weight determination has become more important. It is difficult to determine the absolute molecular weight size due to the lack of precision, whereas the approximate molecular size is determined using HPLC. Therefore, the hydrolysates using acid or enzyme were subjected to precise molecular weight analysis by using Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF). For analysis, two types of matrices were used in this study. The results showed slight differences in sensitivity but were confirmed to be similar to each other. In addition, it was confirmed that any matrix used in this study did not significantly affect the results. In conclusion, the results of this study will provide very important scientific clues in the investigation of the biological activities of chitosan such as antibacterial, anticancer, immune, and antioxidant activity.