Matrix Metalloproteinase-2, Matrix Metalloproteinase-9, and Transforming Growth Factor Beta 1 Levels in Escherichia coli-Infected Rats with Acute Pyelonephritis

Abstract

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and transforming growth factor beta 1 (TGF beta 1) in the kidney tissue of rats with pyelonephritis and their relationship with pyelonephritis by establishing a rat model of acute pyelonephritis. Methods: 80 male Wistar rats were randomly divided into a control group and an experimental group, with 40 rats each. The rats of the control group were injected with and saline and those of the experimental group were injected with 10 ?g/mL Escherichia coli (E. coli) and saline (1:100); the solutions for both groups were administered every 3 days for 7 days. The expressions of MMP-2, MMP-9 and TGF beta 1 in the kidney tissues of rats in each group were observed. Results: The expression of MMP-9 and TGF beta 1in the kidney tissue of rat acute pyelonephritis model rats was significantly higher than those of the control group (P < 0.01); the MMP-9 mRNA content in the kidney tissue of the experimental group was significantly higher than that of the control group (P < 0.05); the TGF beta 1 mRNA content in the renal tissue of the experimental group increased significantly compared to the (P < 0.05); MMP-2, MMP-9 and TGF beta 1 began to express in the early stage of pyelonephritis until the complete formation of renal pelvic edema. The difference between groups was statistically significant (P < 0.01). Conclusion: MMP-9 and TGF beta 1 are important factors regulating renal tubular epithelial cell injury and inflammatory response.