Matrix Metalloproteinase-20 immunolocalization in rat first molar tooth development after treatment with amoxicillin

Abstract

we aimed to apply vaeions solvents and extraction techniques for rice bran amber and jasmine Variety (certified and commercial) to get tricin by using distilled water at boiling point with 70 Cο , distilled water with a 70Cο , ethanol 80% and methanol 80% by maceration extraction with distilled water with 70 Co showed superiority over the other extraction solvents by depending on the concentration of phenols and total flavonoids as total phenols reached of the amber variety (certified and commercial) were 79.82 ±2.95, 79.17 ±2.57 mg/g respectively and flavonoids 0.71 ±0.08 and 0.79 ±0.08 mg/g respectively, was adopted as the extraction solvent in the assistant extraction methods ,soxhlet ,ultrasounication and microwave, rice bran of the commercial jasmine excelled by possessing the highest of total phenols 78.75 ±2.95, 53.19 ±2.06 and 50.91 ±2.47 mg/g respectively and flavonoids 0.87 ±0.20, 0.79 ±0.14 and 0.63 ±0.15 mg/g respectively, therefore rice bran adopted the commercial jasmine for extraction by thiolysis, base, acid, and the thiolysis was the best with the total of phenols 109.82 ±6.41 mg/g and total flavonoids 1.112 ±0.26 mg/g ,the results of separation were shown on a silica gel 60 column identification was proved by HPLC technology the presence of tricin at highest concentration in the thiolysis extract followed by the base hydrolysis reached 23.487 ±1.07and 12.257 ±0.86 µg/ml respectiveluy and was not found in the acid hydrolysis extract tricin gave an anti-inflammatory activity 92.42 ±4.08 % at a concentration 500 mg/ml. Keywords: Rice bran, Tricin, Extraction, Solvent, Flavonoids, Antiinflammatory