Abstract
Gordonia species differentiation is a tedious task. Herein, Gordonia identification was performed according to the standard Bruker score system and a recently proposed score for Gram positive rods identification (≥ 1.5 genus level and ≥ 1.7 species level). New scores significantly improved the identification at genus and species level.
Highlights
The bacterial genus Gordonia currently includes 39 different species (Sowani et al 2017)
Using the 16S rRNA gene sequencing as standard and comparing the manufacturer’s and the new described score system for Gordonia identification, 10 isolates showing an unreliable identification were correctly identified at the genus level (Table 1) with the new system (p < 0.001) and 9 isolates correctly identified at genus level could be identified at the species level (p = 0.006)
None of the 6 R. equi isolates was misidentified as Gordonia in only one case the MALDI-TOF mass spectrometry (MS) identified the isolate as R. equi with a score value of 2.087
Summary
The bacterial genus Gordonia currently includes 39 different species (Sowani et al 2017). This genus is known for its biotechnological applications and it is notable for being an opportunistic human pathogen, causing a broad spectrum of diseases in healthy as well as in immunocompromised individuals (Ramanan et al 2013). The phenotypic identification of Gordonia genus species has been primarily based on biochemical tests (Verma et al 2006). This methodology is timeconsuming and frequently leads to misidentifications. Gene sequencing has become the best technique to ensure a proper identification (Hsueh et al 2014)
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