Abstract
Diffusion-ordered spectroscopy (DOSY) is a powerful tool for investigating mixtures and identifying peaks of chemical components. However, similar diffusion coefficients of the components, particularly for complex mixtures that contain crowded resonances, limit resolution and restrict application of the DOSY technique. In this paper, matrix-assisted DOSY were used to explore whether the diffusion resolution of a complex model involving indole alkaloid mixtures can be realized. Furthermore, we investigated the influence of different factors on the separation effect. The results showed that the changes in diffusion coefficient differences were achieved more obviously when using sodium dodecyl sulfate (SDS) micelles as the matrix. In addition, we also found that increasing the concentration of SDS can improve the resolution of the DOSY spectrum. Finally, after investigating the influence factors and NMR conditions, we demonstrated the applications of the SDS-assisted DOSY on analyzing the total alkaloid extract of Alstonia Mairei, and the virtual separation of mixtures was achieved.
Highlights
Alkaloids are an important class of nitrogen-containing organic compounds, widely existing in natural plants, and are pharmacologically active ingredients in many medicinal plants
It can be seen from the published application research literature that most of the relative change values of the diffusion coefficients are in the tens of percentage points, and a few are only a few percent [35,36,37]
On the basis of applying the Diffusion-ordered spectroscopy (DOSY) technology to the theoretical model mixture, we introduced the technology into the actual mixture to the theoretical model mixture, we introduced the technology into the actual mixture system, using the total alkaloid extract of Alstonia Mairei as the analyte to explore the value system, using the total alkaloid extract of Alstonia Mairei as the analyte to explore the value of sodium dodecyl sulfate (SDS)-assisted DOSY in practical application
Summary
Alkaloids are an important class of nitrogen-containing organic compounds, widely existing in natural plants, and are pharmacologically active ingredients in many medicinal plants. There are various types of alkaloids, among which indole alkaloids are so far the most discovered, and are generally distributed in the Apocynaceae, Loganiaceae, and Rubiaceae families [1,2]. Indole alkaloids are complex in structure, and have significant biological activities [3,4,5], such as anticancer, anti-oxidation, and antibacterial. They are generally used for anticancer [6], antidepressant [7], and neuronal protection [8]. Alstonia mairei is a plant of the genus Alstonia of Apocynaceae, which is rich in monoterpenoid indole alkaloids [9,10]. The ethanol extract from the branches and leaves of Alstonia mairei was found to have antitumor activity
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