Mating type inNeurosporaand closely related ascomycetes: some current problems

Abstract

Neurospora crassa and related ascomycetes such as Podospora anserina exist in two mating types, encoded in a unique region of one chromosome. Classical genetic analysis outlined the nature of the questions and provided important materials for further work. In the mating type region, there is little DNA sequence resemblance between the two mating types. They are, therefore, called idiomorphs rather than alleles. There are no silent copies of these sequences in the genome, so mating type switching is impossible. Cloning, sequence analysis, and complementation studies involving these idiomorphs has begun to shed light on their function. One of the idiomorphs contains three reading frames; one is essential for fertilization and fruiting body formation and the other two are involved in post-fertilization functions including ascus and ascospore formation. In various species of the genus Neurospora, the centromere-proximal flank of the idiomorphs is highly variable in DNA sequence among species, and in some cases, between mating types. The similarities and differences in these flanking sequences allow some conclusions to be drawn about the possible phylogenetic relationship of these species. Key words: Neurospora, ascomycetes, mating, evolution, compatibility, HMG proteins.