Abstract
A simple method is described for construction of mating type heterokaryons in Phycomyces blakesleeanus. The specific morphology of these heterokaryons is used as a phenotypic marker which reveals the presence of nuclei of different mating types in the same mycelium. These heterokaryons can be successfully used in complementation tests involving both nutritional and phototropic markers. Complementation between mutants with abnormal phototropism belonging to seven previously established complementation groups showed that in 54 different mating type heterokaryons the pattern of complementation was the same as that earlier described in heterokaryons involving nuclei of the same mating type. Complementation tests involving riboflavin auxotrophs showed the presence of two complementation groups.
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