Abstract
This chapter describes how to best mate video cameras to microscopes at magnifications that preserve specimen image resolution when necessary. The chapter also considers what needs to be done to maintain the optical corrections of the objective and eyepiece in the video image. Classification of microscope objectives into achromats, fluorites, and apochromats refers to their color and spherical corrections. In a perfect objective, the image does not change its axial location with wavelength. It may, however, change in size. The expectation that an electronic camera will be mated sooner or later to any microscope has profoundly affected the design of contemporary instruments. The C-mount adapter flange has been moved closer to the objective and so intercepts the imaging path in a plane well ahead of the intermediate image. That makes possible the design of couplers that do not compromise optical performance or, in the case of zoom adapters, reduce light transmission significantly.
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