Mating behaviour of Echinostoma caproni and E. trivolvis in concurrent infections in hamsters

Abstract

Young adults of Echinostoma caproni and E. trivolvis were recovered from hamsters. Some worms of each species were exposed to [ 3H]tyrosine for 1 h to label sperm, and these were transplanted singly to uninfected hamsters with various combinations of unexposed worms of either the opposite species or both species. Worms recovered 5 days later were serially sectioned, processed for autoradiography and observed for the location of radioactive sperm. Interspecies mating was detected when E. caproni was the sperm donor and E. trivolvis the recipient, but not the converse. The cross-insemination rate during interspecies mating was very low (13%) when compared to the normal rate of E. caproni intraspecies mating (52%). When single donor adults of either E. caproni or E. trivolvis had a choice of both recipient species, no interspecies mating took place, but both self- and cross-inseminated in a non-restrictive mating pattern typical of echinostome species. After transplantation, both species localized in their normal habitat within the hamster intestine. However, 25% of opposite species recoveries were found within 1 cm of each other, making interspecies mating a possibility.