Mating behavior of the West Nile virus vector Culex pipiens – role of behavioral asymmetries

Abstract

Culex pipiens is a vector of West Nile, Rift Valley fever, Japanese encephalitis and Usutu viruses. In agreement with the criteria of Integrated Vector Management, several research efforts have been devoted to develop behavior-based control tools to fight mosquito vectors. However, our knowledge of mosquito mating biology and sexual communication is still patchy. Despite the high relevance of C. pipiens as a vector of medical and veterinary importance, no studies on its mating behavior and the factors routing mating success have been conducted. In this study, I quantified the mating behavior of an Italian strain of C. pipiens, evaluating the male mating success and its potential connections with population-level lateralized traits occurring during the mating sequence. Mean copula duration exceeded 100 s. Courting males can be straightly accepted by the female after the first genital contact (38.95%), as well as after some rejection kicks performed by females with hind legs (17.89%). No copula duration differences were detected between these two cases. The overall male mating success in laboratory conditions was 56.84%. The females performing rejection kicks preferentially used right hind legs at population-level. This was confirmed over four subsequent testing phases. The number of kicks per rejection event and the rejection success were higher when right legs are used over left ones, showing a functional advantage linked with the employ of right legs. Overall, the present study represents the first quantification of the courtship and mating behavior of C. pipiens. Data on male mating success and the role population-level lateralized mating traits provides basic biological knowledge that can be helpful to optimize autocidal and behavior-based control tools.