Abstract
Cellulase production can be divided into two steps: growth stage; followed by an induction stage. To develop a mathematical model for the optimization of this strategy, two sets of experiments were performed in batch mode for parameter estimation. One set of experiments was performed to evaluate the influence of glycerol regarding cell growth (initial concentrations of 5, 10, 15 and 20 g/L). The other set of experiments considered the induction stage using cellulose as the substrate (initial concentrations of 5, 10, 20, 30 and 40 g/L). Two feeding strategies were simulated to maximize cellulase production using glycerol to maintain a high cell concentration. The first simulation used a discrete feed and the second used a continuous feed of cellulose. The mathematical model proposed allows maintaining a high cell concentration and the addition of optimal small amounts of the inducer substrate to prevent inhibition of enzyme production.
Highlights
In the biotechnological production of biofuels, such as ethanol and other chemicals using lignocellulosic materials, hydrolysis is one of the most important steps of the process
The enzymes used in the hydrolysis step can be produced by filamentous fungi of the genus Trichoderma, which is well adapted for bioprocesses (Strakowska et al, 2014)
The experiment with 15 Glycerol concentration in the feed (g/L) of glycerol was used for validation of the mathematical model using glycerol as the substrate and the experiments with 5 and 40 g/L of cellulose were used for extrapolation analysis of the mathematical model using cellulose as the substrate
Summary
In the biotechnological production of biofuels, such as ethanol and other chemicals using lignocellulosic materials, hydrolysis is one of the most important steps of the process. The importance of cellulase production goes beyond its use in lignocellulose hydrolysis. Recent market reports show that cellulase has increasingly been used in many industrial applications, such as coffee processing, winemaking, fruit juice production, paper and pulping as well as laundry detergents and the production of cleaning and washing agents (Jayasekara and Ratnayake, 2019). These authors cite applications in agriculture and medical area. Separation of the process into two-stage, allowing the optimization of the growth stage and the production stage separately may result in increased process
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