Mathematical modeling and optimization of plasmid-harboring and chromosome-integrated recombinant yeast culture processes

Abstract

For the enhanced secretion of foreign proteins, the mathematical modeling for overall recombinant yeast culture considering protein secretion dynamics is important because we can understand and predict the behavior of biosynthesis and secretion of foreign protein and can develop control strategies for feeding with these model equations. In this research, the mathematical modeling and simulation considering protein secretion dynamics for recombinant yeast cultures that contain multicopy plasmids or chromosomally integrated genes were performed for optimization of foreign glucoamylase production. The optimal feeding policy for maximizing glucoamylase production was suggested in fed-batch culture. By introducing this optimal feeding policy, final glucoamylase activity and productivity of fedbatch culture were significantly increased compared with those of batch culture in both recombinant yeasts.